Fumigation Of Tissue Culture Hoods

Establishing an Orchid Laboratory for Conservation « The RTU Plant ...

Orchids are intriguing plants to grow because of their exotic beauty and mystery. A hobbyist usually starts with nature plants, and after learning the basics of their cultural requirements, will then proceed to grow established seedlings, then to seedling in community pots and maybe up to the ready-to-compot flasks. One of the hobbyist’s is to see this plants grow and multiply. He will then be tempted to grow to his own seedlings. For a hobbyist’s one of the great joys for life is to watch dust-like orchid seeds planted in mother flasks slowly turn green, enlarge, turn into Protocorms and grow into seedlings. Also, having a laboratory will mean that the number of plants you will have will be limited only by your growing space, since orchids produce thousands of seeds. Establishing an orchid laboratory need not to be too expensive nowadays since there are already a lot of substitutes for expensive chemicals and equipment, also there are now a lot of gadgets, glass wares, culture vessels chemicals and equipment to choose form. However a hobbyist’s needs to train first in the basic of orchids micropropagation in order to foresee problems and to know how to trouble shoot them. A trial laboratory could be remodeled inside your home, garage, warehouse, office or a portion of your green house. What is important is the cleanliness of the site, which should be away from source of chemicals (pesticides), dirt and pollution. A correct laboratory design and good practices by the staff usually minimized contamination, the number one problem of most tissue cultured laboratories. Through careful planning it could be made expensive, feasible, applicable and clean. Tissue culture laboratories usually consist of 3 parts, namely (1) a preparation area, (2) a transfer or inoculating room. The order is base on their increasing level of cleanliness with the inner most areawith less foot traffic. In extreme or temporary circumstances, these 3 areas could occupy the same room, but long term operating efficiency and need for cleanliness dictate that each be in a separate room. The second is the transfer room. It is a clean room which contains the transfer chamber. It is in the are where the technician do the inoculation of seeds into mother flasks and sub culturing of Protocorms into reflasking medium. The transfer chamber could be in the form of a laminar flow hood or a still – air inoculating box.

Animal cell culture

Basic cell culture, a practical approach

Principles and Practice of Animal Tissue Culture (Second Edition)

Culture of Animal Cells, A Manual of Basic Technique and Specialized Applications

Reopening public facilities after a biological attack, a decision making framework